Review



cst 13141 antibody  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Cell Signaling Technology Inc cst 13141 antibody
    Mitochondrial reactive oxygen species (ROS) are increased in CPT1A-OE cells. ( A ) Electron paramagnetic resonance spectroscopy (EPR) traces of the four cell lines tested, including the mitochondrial probe by itself. Amplitude and linewidth provide information of the amount of ROS. The concentration was acquired by Spin Fit followed by SpinCount module (Bruker). ( B ) Quantification of the EPR traces normalized to protein content. *** p < 0.001. ( C ) Gene expression analysis of OE versus KD cells for SOD1 ns), <t>SOD2</t> ( p = 6.9 × 10 −4 ), and SOD3 ( p = 0.015). Only SOD2 is a mitochondrial enzyme. ( D , E ) Western blots of SOD2 expression after incubation with fatty acids (Oleic and palmitate mixture; 25 µM each) in FBS ( D ) or charcoal stripped serum (CSS) ( E ) for 48 h. ( F ) Schematic of the role of SOD2 in metabolizing superoxide and the role of glutathione in eliminating H 2 O 2 . GPX = Glutathione peroxide. GSSG = oxidized glutathione.
    Cst 13141 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cst+13141+antibody/cst+13141+antibody/pmc07709014-183-16-17
    Average 90 stars, based on 1 article reviews
    cst 13141 antibody - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "CPT1A Over-Expression Increases Reactive Oxygen Species in the Mitochondria and Promotes Antioxidant Defenses in Prostate Cancer"

    Article Title: CPT1A Over-Expression Increases Reactive Oxygen Species in the Mitochondria and Promotes Antioxidant Defenses in Prostate Cancer

    Journal: Cancers

    doi: 10.3390/cancers12113431

    Mitochondrial reactive oxygen species (ROS) are increased in CPT1A-OE cells. ( A ) Electron paramagnetic resonance spectroscopy (EPR) traces of the four cell lines tested, including the mitochondrial probe by itself. Amplitude and linewidth provide information of the amount of ROS. The concentration was acquired by Spin Fit followed by SpinCount module (Bruker). ( B ) Quantification of the EPR traces normalized to protein content. *** p < 0.001. ( C ) Gene expression analysis of OE versus KD cells for SOD1 ns), SOD2 ( p = 6.9 × 10 −4 ), and SOD3 ( p = 0.015). Only SOD2 is a mitochondrial enzyme. ( D , E ) Western blots of SOD2 expression after incubation with fatty acids (Oleic and palmitate mixture; 25 µM each) in FBS ( D ) or charcoal stripped serum (CSS) ( E ) for 48 h. ( F ) Schematic of the role of SOD2 in metabolizing superoxide and the role of glutathione in eliminating H 2 O 2 . GPX = Glutathione peroxide. GSSG = oxidized glutathione.
    Figure Legend Snippet: Mitochondrial reactive oxygen species (ROS) are increased in CPT1A-OE cells. ( A ) Electron paramagnetic resonance spectroscopy (EPR) traces of the four cell lines tested, including the mitochondrial probe by itself. Amplitude and linewidth provide information of the amount of ROS. The concentration was acquired by Spin Fit followed by SpinCount module (Bruker). ( B ) Quantification of the EPR traces normalized to protein content. *** p < 0.001. ( C ) Gene expression analysis of OE versus KD cells for SOD1 ns), SOD2 ( p = 6.9 × 10 −4 ), and SOD3 ( p = 0.015). Only SOD2 is a mitochondrial enzyme. ( D , E ) Western blots of SOD2 expression after incubation with fatty acids (Oleic and palmitate mixture; 25 µM each) in FBS ( D ) or charcoal stripped serum (CSS) ( E ) for 48 h. ( F ) Schematic of the role of SOD2 in metabolizing superoxide and the role of glutathione in eliminating H 2 O 2 . GPX = Glutathione peroxide. GSSG = oxidized glutathione.

    Techniques Used: Electron Paramagnetic Resonance, Spectroscopy, Concentration Assay, Gene Expression, Western Blot, Expressing, Incubation

    Related Articles

    Electron Paramagnetic Resonance:

    Article Title: CPT1A Over-Expression Increases Reactive Oxygen Species in the Mitochondria and Promotes Antioxidant Defenses in Prostate Cancer
    Article Snippet: Antibodies: CPT1A: 15184-1-AP, (Proteintech, Rosemont, IL, USA); GAPDH: CST 5174, (Cell Signaling Technology, Beverly, MA, USA); SOD2: CST 13141, Cell Signaling Technology.

    Spectroscopy:

    Article Title: CPT1A Over-Expression Increases Reactive Oxygen Species in the Mitochondria and Promotes Antioxidant Defenses in Prostate Cancer
    Article Snippet: Antibodies: CPT1A: 15184-1-AP, (Proteintech, Rosemont, IL, USA); GAPDH: CST 5174, (Cell Signaling Technology, Beverly, MA, USA); SOD2: CST 13141, Cell Signaling Technology.

    Concentration Assay:

    Article Title: CPT1A Over-Expression Increases Reactive Oxygen Species in the Mitochondria and Promotes Antioxidant Defenses in Prostate Cancer
    Article Snippet: Antibodies: CPT1A: 15184-1-AP, (Proteintech, Rosemont, IL, USA); GAPDH: CST 5174, (Cell Signaling Technology, Beverly, MA, USA); SOD2: CST 13141, Cell Signaling Technology.

    Gene Expression:

    Article Title: CPT1A Over-Expression Increases Reactive Oxygen Species in the Mitochondria and Promotes Antioxidant Defenses in Prostate Cancer
    Article Snippet: Antibodies: CPT1A: 15184-1-AP, (Proteintech, Rosemont, IL, USA); GAPDH: CST 5174, (Cell Signaling Technology, Beverly, MA, USA); SOD2: CST 13141, Cell Signaling Technology.

    Western Blot:

    Article Title: CPT1A Over-Expression Increases Reactive Oxygen Species in the Mitochondria and Promotes Antioxidant Defenses in Prostate Cancer
    Article Snippet: Antibodies: CPT1A: 15184-1-AP, (Proteintech, Rosemont, IL, USA); GAPDH: CST 5174, (Cell Signaling Technology, Beverly, MA, USA); SOD2: CST 13141, Cell Signaling Technology.

    Expressing:

    Article Title: CPT1A Over-Expression Increases Reactive Oxygen Species in the Mitochondria and Promotes Antioxidant Defenses in Prostate Cancer
    Article Snippet: Antibodies: CPT1A: 15184-1-AP, (Proteintech, Rosemont, IL, USA); GAPDH: CST 5174, (Cell Signaling Technology, Beverly, MA, USA); SOD2: CST 13141, Cell Signaling Technology.

    Incubation:

    Article Title: CPT1A Over-Expression Increases Reactive Oxygen Species in the Mitochondria and Promotes Antioxidant Defenses in Prostate Cancer
    Article Snippet: Antibodies: CPT1A: 15184-1-AP, (Proteintech, Rosemont, IL, USA); GAPDH: CST 5174, (Cell Signaling Technology, Beverly, MA, USA); SOD2: CST 13141, Cell Signaling Technology.



    Similar Products

    cst 13141 antibody  (Cell Signaling Technology Inc)
    90
    Cell Signaling Technology Inc cst 13141 antibody
    Mitochondrial reactive oxygen species (ROS) are increased in CPT1A-OE cells. ( A ) Electron paramagnetic resonance spectroscopy (EPR) traces of the four cell lines tested, including the mitochondrial probe by itself. Amplitude and linewidth provide information of the amount of ROS. The concentration was acquired by Spin Fit followed by SpinCount module (Bruker). ( B ) Quantification of the EPR traces normalized to protein content. *** p < 0.001. ( C ) Gene expression analysis of OE versus KD cells for SOD1 ns), <t>SOD2</t> ( p = 6.9 × 10 −4 ), and SOD3 ( p = 0.015). Only SOD2 is a mitochondrial enzyme. ( D , E ) Western blots of SOD2 expression after incubation with fatty acids (Oleic and palmitate mixture; 25 µM each) in FBS ( D ) or charcoal stripped serum (CSS) ( E ) for 48 h. ( F ) Schematic of the role of SOD2 in metabolizing superoxide and the role of glutathione in eliminating H 2 O 2 . GPX = Glutathione peroxide. GSSG = oxidized glutathione.
    Cst 13141 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cst+13141+antibody/cst+13141+antibody/pmc07709014-183-16-17
    Average 90 stars, based on 1 article reviews
    cst 13141 antibody - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Mitochondrial reactive oxygen species (ROS) are increased in CPT1A-OE cells. ( A ) Electron paramagnetic resonance spectroscopy (EPR) traces of the four cell lines tested, including the mitochondrial probe by itself. Amplitude and linewidth provide information of the amount of ROS. The concentration was acquired by Spin Fit followed by SpinCount module (Bruker). ( B ) Quantification of the EPR traces normalized to protein content. *** p < 0.001. ( C ) Gene expression analysis of OE versus KD cells for SOD1 ns), SOD2 ( p = 6.9 × 10 −4 ), and SOD3 ( p = 0.015). Only SOD2 is a mitochondrial enzyme. ( D , E ) Western blots of SOD2 expression after incubation with fatty acids (Oleic and palmitate mixture; 25 µM each) in FBS ( D ) or charcoal stripped serum (CSS) ( E ) for 48 h. ( F ) Schematic of the role of SOD2 in metabolizing superoxide and the role of glutathione in eliminating H 2 O 2 . GPX = Glutathione peroxide. GSSG = oxidized glutathione.

    Journal: Cancers

    Article Title: CPT1A Over-Expression Increases Reactive Oxygen Species in the Mitochondria and Promotes Antioxidant Defenses in Prostate Cancer

    doi: 10.3390/cancers12113431

    Figure Lengend Snippet: Mitochondrial reactive oxygen species (ROS) are increased in CPT1A-OE cells. ( A ) Electron paramagnetic resonance spectroscopy (EPR) traces of the four cell lines tested, including the mitochondrial probe by itself. Amplitude and linewidth provide information of the amount of ROS. The concentration was acquired by Spin Fit followed by SpinCount module (Bruker). ( B ) Quantification of the EPR traces normalized to protein content. *** p < 0.001. ( C ) Gene expression analysis of OE versus KD cells for SOD1 ns), SOD2 ( p = 6.9 × 10 −4 ), and SOD3 ( p = 0.015). Only SOD2 is a mitochondrial enzyme. ( D , E ) Western blots of SOD2 expression after incubation with fatty acids (Oleic and palmitate mixture; 25 µM each) in FBS ( D ) or charcoal stripped serum (CSS) ( E ) for 48 h. ( F ) Schematic of the role of SOD2 in metabolizing superoxide and the role of glutathione in eliminating H 2 O 2 . GPX = Glutathione peroxide. GSSG = oxidized glutathione.

    Article Snippet: Antibodies: CPT1A: 15184-1-AP, (Proteintech, Rosemont, IL, USA); GAPDH: CST 5174, (Cell Signaling Technology, Beverly, MA, USA); SOD2: CST 13141, Cell Signaling Technology.

    Techniques: Electron Paramagnetic Resonance, Spectroscopy, Concentration Assay, Gene Expression, Western Blot, Expressing, Incubation